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991.
吖啶橙失活处理应用于不对称融合初步探讨 总被引:1,自引:0,他引:1
马铃薯栽培种和野生种叶肉原生质体经过吖啶橙(AO)处理后,暗培养两天,再光照4h而失活.失活程度取决于AO处理时间、AO浓度、光照时机等.四倍体失活浓度高于二倍体,处理后马上光照失活效果最强烈,8d后光照其小细胞团同未光照处理相差不大。AO失活的栽培种NO7同罗丹明6G(R6G)失活的Solanumbulbocastanum融合后可以发生代谢互补恢复分裂,已得到假定的杂种小愈伤组织. 相似文献
992.
马铃薯野生种叶肉原生质体培养及其植株再生(简报)何亚文,李耿光,张兰英(中国科学院华南植物研究所,广州510650)MESOPHYLLPROTOPLASTCULTUREANDPLANTLETREGENERATIONFROMWILDSPECIESOFP... 相似文献
993.
通过作在昆虫生物学方面近五十年研究工作的体会.提出了组建昆虫系统生物学的目的性和可行性探索.拟以昆虫分类序列目、科、属、种为单元,将一些已知种类的有关生物学环节.一一列叙.编成《中国农林昆虫系统生物学手册》.再以目、科、属、种为主体,探求彼此间在生物学各环节间的共性和个性,分别加以比较、归纳和分析.作为这—分支学科的蘸本。 相似文献
994.
本记述了采自西藏小型动物体上的恙螨四新种.隶属恙螨科(Trombiculidae Ewing,1944)的纤恙螨属(Leptotrombitium Nagyio et al,1916)。模式标本保存于军事医学科学院微生物流行病学研究所医学昆虫标本馆,部分副模标本存第一作所在单位。 相似文献
995.
N-甲基甲酰胺碱度是提取高质量固氮酶铁钼辅基的关键因素之一。过量的亚甲蓝能氧化并分解铁铜铺基为含双相铁硫簇和铁硫簇固氮酶铁钼辅基和在紫外可见光谱区中均无特征吸收峰,而在320nm处却呈弱吸收峰,棕色固氮菌固氮酶和该菌的突变菌侏UW45固氮酶(缺铁钼辅基)中的非含钼的铁硫簇在紫外可见光谱区320nm和405nm处均含有特征吸收峰. 相似文献
996.
Forskolin(FSK)是一种植物二萜类化合物,为腺苷酸环化酶的特异激活剂,实验发现:FSK和作为参照的诱导分化剂维甲酸(RA)单独或联合应用均可升高胞浆蛋白激酶C(PKC)活性,并降低膜PKC活性,FSK可使表皮生长因子(EGF)诱导的细胞内三磷酸肌醇(IP3-1,4,5)水平降低至对照组的44.4%至67%;FSK与RA合用可显著降低成骨样细胞特征蛋白碱性磷酸酶(AKP)的活性。以上结果表明,FSK对成骨样细胞内磷脂酰肌醇信息传递体系有深刻影响,可能与其调节细胞的增殖分化有关。 相似文献
997.
Chen J Zhang LF Han C Yu GS Ma J 《Journal of gravitational physiology : a journal of the International Society for Gravitational Physiology》1996,3(1):57-62
We have previously demonstrated that prolonged simulated microgravity (tail-suspension) leads to cardiac alterations with increased resting heart rate, myocardial degradation changes and attenuated myocardial contractility. The present study investigated the potential role of adrenoceptor mechanisms underlying them. Changes of myocardial alpha 1-adrenoceptor (alpha 1-AR) and beta 1-adrenoceptor (beta-AR) in 90-day tail-suspended rats was investigated by the method of radioligand binding assay and application of Scatchard's method. The results showed significantly decreased quantity of specific binding of 125I-BE[2-beta-(4-hydroxy-3-[125I]indophenyl)-ethylaminomethyltetralone] to alpha 1-AR present in membrane derived from ventricular myocardium of the suspended animals, despite the affinity of the alpha 1-AR to 125I-Be was unchanged. But neither the quantity nor the affinity of beta-AR binding to 125I-Pindolol was significantly altered. In addition, the spontaneously beating rate of isolated right atria from tail-suspended animals showed little change in sensitivity and reactivity to the stimulations of graded phenylephrine (alpha-agonist, measured in the presence of beta-antagonist propranolol) and isoproterenol (beta-agonist), compared with the control rats. There were also no obvious differences of the effects of the isoproterenol on the contractility of isolated left ventricular papillary muscles between the two groups. Since myocardial alpha 1-AR mediated-effects include production of cardiac hypertrophy and enhancement of myocardial glucose uptake and glycolysis, the down-regulation of the alpha 1-AR may be a contributor to the cardiac cellular accumulation and the myocardial degradation changes as found in our tail-suspended rats. The data from this study also suggest that the myocardial beta-adrenoceptors are not affected by the prolonged tail-suspension. 相似文献
998.
Abstract The present paper deals with a new species Habrophlebiodes zijinensis sp. nov. collected in Nanjing, Jiangsu Povince, China. 相似文献
999.
A gene encoding a truncated large subunit of Rubisco is transcribed and salt-inducible in rice 总被引:16,自引:0,他引:16
J.-S. Zhang J. Gu F.-H. Liu S.-Y. Chen 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1995,91(2):361-366
Using the rice salt-tolerant mutant 20 as material, a cDNA library was constructed and two salt-inducible clones, SIR5.5 and SIR8.1, were isolated by differential screening. Homology analysis revealed that the two clones together constituted a chimeric rbcL which encoded a truncated large subunit of Rubisco with 337 amino-acids, plus 64 amino-acids of unknown origin. The expressions of both the normal and the chimeric locus appeared to be developmentally regulated and salt-inducible in shoots of the salt-tolerant mutant 20 and its original variety 77–170. In roots, their expressions were salt-inducible in the salt-tolerant mutant 20 whereas no, or only premature, forms were present in the salt-treated original variety 77–170. Higher concentrations of salt reduced the expressions of both normal rbcL and the chimeric locus. ABA showed no effect on their expression. 相似文献
1000.
P. Wu G. Zhang J. K. Ladha S. R. McCouch N. Huang 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1995,91(8):1177-1183
An F2 population, consisting of 231 individuals derived from a cross between rice cultivars with a similar growing duration, Palawan and IR42, was utilized to investigate the genetic nature of rice varietal ability to stimulate N2 fixation in the rice rhizosphere. To assess rhizospheric N2 fixation, an isotope-enriched 15N dilution technique was employed, using 15N-stabilized soil in pots. IR42, an indica variety, had 23% higher N derived from fixation (Ndfa) than Palawan, a javanica genotype. Normal segregation of atom% 15N excess was obtained in the F2 population, with an average of 0.218 with 8% of plants below IR42 (0.188) and 10% of plants above Palawan (0.248). One-hundred-and-four RFLP markers mapped on 12 chromosomes were tested for linkage to the putative QTLs. Significant (P<0.01) associations between markers and segregation of atom% 15N excess were observed for seven marker loci located on chromosomes 1, 3, 6 and 11. Four QTLs defined by the detected marker loci were identified by interval-mapping analysis. Additive gene action was found to be predominant, but for at least one locus, dominance and partial dominance effects were observed. Significant (P<0.01) epistatic effects were also identified. Individual marker loci detected between 8 and 16% of the total phenotypic variation. All four putative QTLs showed recessive gene action, and no phenotypic effects associated with heterozygosity of marker loci were observed. The results of this study suggest that rice genetic factors can be identified which affect levels of atom% 15N excess in the soil by interacting with diazotrophs in the rice rhizosphere. 相似文献